Gohan used the attack during his battle against Super Buu w/ Gotenks. After exhanging several blows with Super Buu, Gohan jumped in the air off a few boulders and fired the Ultimate Kamehameha down at Super Buu, who countered the attack with a Vanishing Beam. The two energy waves collided in an energy beam struggle and neither attack was able to gain the upper hand. When Gohan and Super Buu charged more power into the Ultimate Kamehameha and Vanishing Beam respectively, the beam struggle grew bigger until it ended with a huge explosion.
The attack is seen in the Budokai Tenkaichi series, where it is blue-colored and one of Ultimate Gohan's Blast 2 attacks, under the name, "Super Kamehameha." The attack also appeared in Dragon Ball GT: Final Bout as one of Gohan's attacks. In this game, its color is golden-yellow, the same color in the anime.